FASTK antibody

• Catalog Number: orb215025
• Category: Antibodies
• Description: Rabbit polyclonal antibody to FASTK
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: IF, WB
• Reactivity: Human, Mouse, Porcine, Rat
• Immunogen: KLH-conjugated synthetic peptide encompassing a sequence within the center region of human FAST Kinase. The exact sequence is proprietary.
• Dilution range: WB: 1-500-1-1000, IF/ICC: 1-100-1-500
• Form/Appearance: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
• Conjugation: Unconjugated
• Target: FASTK
• Entrez: 10922
• UniProt ID: Q14296
• Source: Rabbit
• Storage: Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.
• Buffer/Preservatives: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
• Alternative names: Fas-activated serine/threonine kinase; FAST kinase
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

Western blot analysis of FAST Kinase expression in HEK293T (A), H460 (B), mouse brain (C), mouse heart (D), rat heart (E) whole cell lysates. (Predicted band size: 61 kD; Observed band size: 61 kD)

Immunofluorescent analysis of FAST Kinase staining in HL60 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark.