FAS monoclonal antibody (M01), clone 5B4

• Catalog Number: orb2295946
• Category: Antibodies
• Description: Mouse monoclonal antibody raised against a partial recombinant FAS.
• Species/Host: Mouse
• Clonality: Monoclonal
• Clone Number: 5B4
• Tested applications: ELISA, IP, WB
• Reactivity: Human
• Isotype: IgG1 Kappa
• Immunogen: FAS (NP_000034, 20 a.a. ~ 119 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
• Conjugation: Unconjugated
• Protein Sequence: SKSVNAQVTDINSKGLELRKTVTTVETQNLEGLHHDGQFCHKPCPPGERKARDCTVNGDEPDCVPCQEGKEYTDKAHFSSKCRRCRLCDEGHGLEVEINC
• NCBI: NP_000034
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: In 1x PBS, pH 7.4
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

Detection limit for recombinant GST tagged FAS is 3 ng/ml as a capture antibody.

Immunoprecipitation of FAS transfected lysate using anti-FAS monoclonal antibody and Protein A Magnetic Bead, and immunoblotted with FAS MaxPab rabbit polyclonal antibody.

Western Blot analysis of FAS expression in transfected 293T cell line by FAS monoclonal antibody (M01), clone 5B4. Lane 1: FAS transfected lysate(37.7 KDa). Lane 2: Non-transfected lysate.

Western Blot detection against Immunogen (36.74 KDa).