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Catalog Number | orb639743 |
---|---|
Category | Antibodies |
Description | This MAb specifically recognizes CD95, also known as Fas, a transmembrane glycoprotein with a MW of 40-45kDa, containing 8kDa of N-glycoside-linked polysaccharide. It is a receptor for TNFSF6/FASLG, a member of the nerve growth factor receptor/tumor necrosis factor superfamily, mediating receptor-triggered apoptosis. The adapter molecule FADD recruits caspase-8 to the activated receptor. The resulting death-inducing signaling complex (DISC) performs caspase-8 proteolytic activation, which initiates the subsequent cascade of caspases (aspartate-specific cysteine proteases) mediating apoptosis. FAS-mediated apoptosis may have a role in the induction of peripheral tolerance, in the antigen-stimulated suicide of mature T-cells, or both. The secreted isoforms 2 to 6 block apoptosis (in vitro). CD95 antigen is expressed on the surface of various cell types, preferentially on the CD45RAlow CD45ROhigh subset of memory T lymphocytes. |
Species/Host | Mouse |
Clonality | Monoclonal |
Clone Number | FAS/3588 |
Tested applications | ELISA, IHC-P |
Reactivity | Human |
Isotype | Mouse IgG2b, kappa |
Immunogen | A human recombinant partial protein (amino acids 26-96) was used as the immunogen for the CD95 antibody. |
Dilution range | ELISA (order BSA-free format for coating),Immunohistochemistry (FFPE): 1-2ug/ml |
Purity | Protein G affinity chromatography |
Conjugation | Unconjugated |
Formula | 0.2 mg/ml with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide |
Hazard Information | This CD95 antibody is available for research use only. |
UniProt ID | P25445 |
Storage | Store the CD95 antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide). |
Buffer/Preservatives | 0.2 mg/ml with 0.1 mg/ml rAlbumin (US sourced), 0.05% sodium azide |
Note | For research use only |
Application notes | Optimal dilution of the antibody should be determined by the researcher. |
Expiration Date | 12 months from date of receipt. |
FACS, Functional Studies, IF, IHC-Fr | |
Human | |
Mouse | |
Monoclonal | |
Unconjugated |
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