FANCA Antibody

• Catalog Number: orb1527443
• Category: Antibodies
• Description: FANCA Antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: FC, IP, WB
• Reactivity: Human
• Immunogen: Between 1405 and 1455
• Concentration: 200 µg/ml
• Dilution range: WB - 1:2,000 - 1:10,000; IP - 2 - 10 µg/mg lysate
• Form/Appearance: Whole IgG
• Conjugation: Unconjugated
• Target: FANCA
• UniProt ID: O15360
• NCBI: NP_000126.2
• Storage: 2 - 8°C
• Buffer/Preservatives: Tris-buffered Saline containing 0.1% rAlbumin and 0.09% Sodium Azide
• Alternative names: Fanconi anemia, type 1; FA; Protein FACA; Fanconi
• Note: For research use only
• Application notes: Format: Whole IgG
• Expiration Date: 12 months from date of receipt.

Detection of human FANCA by western blot. Samples: Whole cell lysate (50 µg) from HeLa, K-562, A-549, MCF-7, and HEK293T cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-FANCA antibody (orb1527443) used for WB at 0.04 µg/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.

Detection of human FANCA by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from HeLa cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-FANCA antibody (orb1527443) used for IP at 6 µg per reaction.

Detection of human FANCA (shaded) in HDLM-2 cells by flow cytometry.Antibody: Rabbit anti-FANCA polyclonal antibody (orb1527443) or isotype control (unshaded). Secondary: DyLight® 488-conjugated goat anti-rabbit IgG.