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Catalog Number | orb764367 |
---|---|
Category | Antibodies |
Description | Rabbit polyclonal antibody to FAK (phospho-Tyr397) |
Species/Host | Rabbit |
Clonality | Polyclonal |
Tested applications | ELISA, IHC-P, WB |
Reactivity | Human, Mouse, Rat |
Isotype | IgG |
Immunogen | The antiserum was produced against synthesized peptide derived from human FAK around the phosphorylation site of Tyr397. AA range:363-412 |
Concentration | 1 mg/ml |
Conjugation | Unconjugated |
MW | 119 |
Storage | Store at -20°C. Avoid repeated freeze-thaw cycles. |
Buffer/Preservatives | PBS with 0.02% sodium azide and 50% glycerol pH 7.4. |
Alternative names | Anti-PTK2 antibody, anti-FAK antibody, anti-FAK1 a Read more... |
Note | For research use only |
Expiration Date | 12 months from date of receipt. |
Western blot analysis of lysates from 1) 293T, 2) AD293 , 3) Hela cells, was diluted at 1:10000, 37° 1hour. antibody was diluted at 1:5000 as loading control, 4°C over night, secondary antibody was diluted at 1:10000, 37° 1hour.
Immunohistochemical analysis of paraffin-embedded Human-liver tissue. 1, FAK (phospho Tyr397) Polyclonal Antibody was diluted at 1:200 (4°C, overnight). 2, Sodium citrate pH 6.0 was used for antibody retrieval (>98°C, 20min). 3, Secondary antibody was diluted at 1:200 (room temperature, 30min). Negative control was used by secondary antibody only.
Immunohistochemical analysis of paraffin-embedded Human-stomach-cancer tissue. 1, FAK (phospho Tyr397) Polyclonal Antibody was diluted at 1:200 (4°C, overnight). 2, Sodium citrate pH 6.0 was used for antibody retrieval (>98°C, 20min). 3, Secondary antibody was diluted at 1:200 (room temperature, 30min). Negative control was used by secondary antibody only.
Immunohistochemical analysis of paraffin-embedded Mouse-brain tissue. 1, FAK (phospho Tyr397) Polyclonal Antibody was diluted at 1:200 (4°C, overnight). 2, Sodium citrate pH 6.0 was used for antibody retrieval (>98°C, 20min). 3, Secondary antibody was diluted at 1:200 (room temperature, 30min). Negative control was used by secondary antibody only.
ELISA, WB | |
Human, Mouse, Rat | |
Rabbit | |
Polyclonal | |
Unconjugated |
IHC-P, WB | |
Bovine, Canine, Equine, Gallus, Rabbit |
WB | |
Human, Mouse, Rat | |
Rabbit | |
Polyclonal | |
Unconjugated |
WB | |
Human, Mouse, Rat | |
Rabbit | |
Recombinant | |
Unconjugated |
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