Factor VIII antibody

• Catalog Number: orb213914
• Category: Antibodies
• Description: Rabbit polyclonal antibody to Factor VIII
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: IH, WB
• Reactivity: Human
• Immunogen: KLH-conjugated synthetic peptide encompassing a sequence within the C-term region of human Factor VIII. The exact sequence is proprietary.
• Dilution range: WB: 1-500-1-1000
• Form/Appearance: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
• Conjugation: Unconjugated
• Target: F8
• Entrez: 2157
• UniProt ID: P00451
• Source: Rabbit
• Storage: Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.
• Buffer/Preservatives: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
• Alternative names: Anti-F8 antibody, anti-F8b antibody, anti-F8c anti
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

Western blot analysis of Factor VIII expression in HeLa (A), HepG2 (B) whole cell lysates. (Predicted band size: 267 kD; Observed band size: 280 kD)

Immunohistochemical analysis of Factor VIII staining in human brain formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.