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Catalog Number | orb371669 |
---|---|
Category | Antibodies |
Description | Factor I/CFI Antibody |
Species/Host | Rabbit |
Clonality | Polyclonal |
Tested applications | FC, ICC, IHC, WB |
Reactivity | Human, Rat |
Isotype | Rabbit IgG |
Immunogen | E. coli-derived human Factor I recombinant protein (Position: K19-D220). Human Factor I shares 70.7% and 71.2% amino acid (aa) sequence identity with mouse and rat Factor I, respectively. |
Concentration | Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml. |
Dilution range | Western blot, 0.1-0.5μg/ml, Human, RatImmunohistochemistry (Frozen Section), 0.5-1μg/ml, Human Immunocytochemistry, 0.5-1μg/ml, Human Flow Cytometry, 1-3μg/1x106 cells, Human |
Form/Appearance | Lyophilized |
Conjugation | Unconjugated |
MW | 65750 MW |
UniProt ID | P05156 |
Storage | Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles. |
Alternative names | Complement factor I;3.4.21.45;C3B/C4B inactivator; Read more... |
Note | For research use only |
Application notes | Tested Species: In-house tested species with positive results. Other applications have not been tested. Optimal dilutions should be determined by end users. . Add 0.2ml of distilled water will yield a concentration of 500ug/ml. |
Expiration Date | 12 months from date of receipt. |
Flow Cytometry analysis of U-87 cells using anti-Factor I antibody (Blue line).Isotype control antibody (Green line) was rabbit IgG .Unlabelled sample (Red line) was also used as a control.
Flow Cytometry analysis of HEPG2 cells using anti-Factor I antibody (Blue line).Isotype control antibody (Green line) was rabbit IgG .Unlabelled sample (Red line) was also used as a control.
WB analysis of Factor I using anti-Factor I antibody.Lane 1:rat liver tissue;2:HELA cell.
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