FABP5 Antibody (epidermal)

• Catalog Number: orb2641476
• Category: Antibodies
• Description: This gene encodes the fatty acid binding protein found in epidermal cells, and was first identified as being upregulated in psoriasis tissue. Fatty acid binding proteins are a family of small, highly conserved, cytoplasmic proteins that bind long-chain fatty acids and other hydrophobic ligands. FABPs may play roles in fatty acid uptake, transport, and metabolism. Polymorphisms in this gene are associated with type 2 diabetes. The human genome contains many pseudogenes similar to this locus. FABP5 is also associated with poor survival in triple-negative breast cancer. Additionally, FABP5 gene is upregulated in colorectal cancer cells compared to normal colon cells in a manner that correlates with disease stage and that FABP5 significantly promotes colorectal cancer cell growth and metastatic potential.
• Species/Host: Mouse
• Clonality: Monoclonal
• Clone Number: CPTC-FABP5-3
• Tested applications: IHC-P, WB
• Reactivity: Human
• Isotype: Mouse IgG2a, kappa
• Immunogen: Recombinant full length human protein was used as the immunogen for the FABP5 antibody.
• Antibody Type: Primary Antibody
• Dilution range: Western blot: 1-2ug/ml,Immunohistochemistry (FFPE): 1-2ug/ml for 30 min at RT
• Purity: Protein G affinity chromatography
• Conjugation: Unconjugated
• Formula: 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide
• Hazard Information: This FABP5 antibody is available for research use only.
• UniProt ID: Q01469
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: 0.2 mg/ml in 1X PBS with 0.1 mg/ml rAlbumin (US sourced) and 0.05% sodium azide
• Note: For research use only
• Application notes: Titering of the FABP5 antibody may be required for optimal performance.1. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.
• Expiration Date: 12 months from date of receipt.

IHC testing of FFPE human hepatic carcinoma with FABP5 antibody. HIER: boiling tissue sections in pH9 10mM Tris with 1mM EDTA for 10-20 min and allow to cool prior to staining.

IHC testing of FFPE human skeletal muscle with FABP5 antibody. HIER: boiling tissue sections in pH9 10mM Tris with 1mM EDTA for 10-20 min and allow to cool prior to staining.

Western blot testing of human HeLa cell lysate with FABP5 antibody. Predicted molecular weight ~15 kDa.