FABP2 antibody

• Catalog Number: orb19465
• Category: Antibodies
• Description: Goat polyclonal antibody to FABP2
• Species/Host: Goat
• Clonality: Polyclonal
• Tested applications: ELISA, FC, IHC, WB
• Reactivity: Bovine, Canine, Human, Mouse, Porcine, Rat
• Dilution range: ELISA: 1:64000, WB: 0.001 μg/ml, IHC-P: 2-3 μg/ml
• Conjugation: Unconjugated
• MW: 15.2
• Target: FABP2
• Entrez: 2169
• Protein Sequence: EGVEAKRIFKKD
• Storage: Aliquot and store at -20°C. Minimize freezing and thawing.
• Buffer/Preservatives: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
• Alternative names: anti FABP2 antibody, anti fatty acid binding prote
• Note: For research use only
• Application notes: ELISA: Peptide ELISA: antibody detection limit dilution 1:64000.IHC: In paraffin embedded Human Small Intestine shows strong staining of basement membrane and weaker staining of enterocytes. Recommended concentration, 2-3ug/ml.WB: A very strong approx 16kDa band observed in Human Duodenum lysates (calculated MW of 15.2kDa according to NP_000125.1). In transfected HEK293 transiently expressing FABP2 a band of approx. 18kDa is observed. This band is not observed in the non-transfected HEK293. Recommended concentration: 0.001 μg/ml.
• Expiration Date: 12 months from date of receipt.

HEK293 overexpressing FABP2 (RC210206) and probed with orb19465 (mock transfection in first lane), tested by Origene.

orb19465 (2.5ug/ml) staining of paraffin embedded Human Small Intestine. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.

orb19465 (0.001µg/ml) staining of Human Duodenum lysate (35µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.