FABP1 Antibody

• Catalog Number: orb2636533
• Category: Antibodies
• Description: Fatty acid-binding proteins, designated FABPs, are a family of homologous cytoplasmic proteins that are expressed in a highly tissue-specific manner and play an integral role in the balance between lipid and carbohydrate metabolism. FABPs mediate fatty acid (FA) and/or hydrophobic ligand uptake, transport and targeting within their respective tissues. The mechanisms underlying these actions can give rise to both passive diffusional uptake and protein-mediated transmembrane transport of FAs. FABPs are expressed in adipocytes (A-FABP), brain (B-FABP), epithelium (E-FABP, psoriasis-associated FABP, PA-FABP), striated muscle and heart (H-FABP, mammary-derived growth inhibitor or MDGI), intestine (I-FABP), liver (L-FABP or FABP1), myelin (M-FABP) and testis (T-FABP). FABP1 (L-FABP) expression is modulated by developmental, hormonal, dietary and pharmacological factors, and is required for cholesterol synthesis and metabolism.
• Species/Host: Mouse
• Clonality: Monoclonal
• Clone Number: FABP1/3940
• Tested applications: IHC-P, WB
• Reactivity: Human
• Isotype: Mouse IgG2b, kappa
• Immunogen: A portin of amino acids 1-127 from the human protein was used as the immunogen for the FABP1 antibody.
• Antibody Type: Primary Antibody
• Dilution range: Western blot: 2-4ug/ml,Immunohistochemistry (FFPE): 1-2ug/ml
• Purity: Protein A/G affinity
• Conjugation: Unconjugated
• Formula: 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide
• Hazard Information: This FABP1 antibody is available for research use only.
• UniProt ID: P07148
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: 0.2 mg/ml in 1X PBS with 0.1 mg/ml rAlbumin (US sourced), 0.05% sodium azide
• Note: For research use only
• Application notes: Optimal dilution of the FABP1 antibody should be determined by the researcher.
• Expiration Date: 12 months from date of receipt.

IHC staining of FFPE human kidney tissue with FABP1 antibody (clone FABP1/3940). HIER: boil tissue sections in pH9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.

IHC staining of FFPE human kidney tissue with FABP1 antibody (clone FABP1/3940). HIER: boil tissue sections in pH9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.

SDS-PAGE analysis of purified, BSA-free FABP1 antibody (clone FABP1/3940) as confirmation of integrity and purity.

Western blot testing of human kidney tissue lysate using FABP1 antibody (clone FABP1/3940). Predicted molecular weight ~14 kDa.

Analysis of HuProt (TM) microarray containing more than 19000 full-length human proteins using FABP1 antibody (clone FABP1/3940). These results demonstrate the foremost specificity of the FABP1/3940 mAb. Z- and S- score: The Z-score represents the strength of a signal that an antibody (in combination with a fluorescently-tagged anti-IgG secondary Ab) produces when binding to a particular protein on the HuProt (TM) array. Z-scores are described in units of standard deviations (SD's) above the mean value of all signals generated on that array. If the targets on the HuProt (TM) are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD's) between the Z-scores. The S-score therefore represents the relative target specificity of an Ab to its intended target.