F(ab')2 Human kappa (kappa chain) antibody (Peroxidase)

• Catalog Number: orb348186
• Category: Antibodies
• Description: F(ab')2 Human kappa (kappa chain) antibody (Peroxidase)
• Species/Host: Goat
• Clonality: Polyclonal
• Tested applications: ELISA, IHC, WB
• Reactivity: Human
• Isotype: IgG F(ab')2
• Immunogen: Human kappa light chain
• Concentration: 1.0 mg/mL
• Dilution range: ELISA: 1:50,000, IHC: 1:1,000 - 1:5,000, WB: 1:2,000 - 1:10,000
• Form/Appearance: Lyophilized
• Purity: F(ab')2 Anti-Human (kappa chain) Antibody was prepared from monospecific antiserum by immunoaffinity chromatography using Human antigens coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities, pepsin digestion and chromatographic separation. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Peroxidase and anti-Goat Serum. No reaction was observed against anti-Pepsin or anti-Goat IgG F(c). Specificity was confirmed by ELISA at less than 1% cross reactivity against other human heavy or light chain isotypes.
• Conjugation: HRP
• Storage: Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
• Buffer/Preservatives: 0.01% (w/v) Thimerosal
• Alternative names: Goat F(ab')2 Anti-Human κ (kappa chain) Antibody P
• Note: For research use only
• Application notes: F(ab')2 anti-Human (kappa chain) Peroxidase Conjugated Antibody has been tested by ELISA and is assayed against 1.0 ug of human kappa light chain in a standard capture ELISA using ABTS (2,2’-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) code # ABTS-100 as a substrate for 30 minutes at room temperature. A working dilution of 1:2,000 to 1:8,000 of the reconstitution concentration is suggested for this product.
• Expiration Date: 12 months from date of receipt.