F2 monoclonal antibody (M01), clone 1B7

• Catalog Number: orb2293145
• Category: Antibodies
• Description: Mouse monoclonal antibody raised against a partial recombinant F2.
• Species/Host: Mouse
• Clonality: Monoclonal
• Clone Number: 1B7
• Tested applications: ELISA, IP, PLA, WB
• Reactivity: Human
• Isotype: IgG
• Immunogen: F2 (AAH51332, 263 a.a. ~ 362 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
• Conjugation: Unconjugated
• Protein Sequence: RNPDGDEEGAWCYVAGKPGDFGYCDLNYCEEAVEEETGDGLDEDSDRAIEGRTATSEYQTFFNPRTFGSGEADCGLRPLFEKKSLEDKTERELLESYIDG
• NCBI: AAH51332
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: In 1x PBS, pH 7.4
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

Detection limit for recombinant GST tagged F2 is approximately 1 ng/ml as a capture antibody.

Immunoprecipitation of F2 transfected lysate using anti-F2 monoclonal antibody and Protein A Magnetic Bead, and immunoblotted with F2 MaxPab rabbit polyclonal antibody.

Proximity Ligation Analysis of protein-protein interactions between FGA and F2. HeLa cells were stained with anti-FGA rabbit purified polyclonal 1:1200 and anti-F2 mouse monoclonal antibody 1:50. Each red dot represents the detection of protein-protein interaction complex, and nuclei were counterstained with DAPI (blue).

Western Blot analysis of F2 expression in transfected 293T cell line by F2 monoclonal antibody (M01), clone 1B7. Lane 1: F2 transfected lysate (70 KDa). Lane 2: Non-transfected lysate.

Western blot analysis of F2 over-expressed 293 cell line, cotransfected with F2 Validated Chimera RNAi (Lane 2) or non-transfected control (Lane 1). Blot probed with F2 monoclonal antibody (M01), clone 1B7 (Cat # orb2293145). GAPDH (36.1 kDa) used as specificity and loading control.

Western Blot detection against Immunogen (36.74 KDa).