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Catalog Number | orb2293131 |
---|---|
Category | Antibodies |
Description | Mouse monoclonal antibody raised against a partial recombinant F11. |
Species/Host | Mouse |
Clonality | Monoclonal |
Clone Number | 2H8 |
Tested applications | ELISA, IP, WB |
Reactivity | Human |
Isotype | IgG1 Kappa |
Immunogen | F11 (NP_000119, 286 a.a. ~ 385 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. |
Conjugation | Unconjugated |
Protein Sequence | SIPVFCHSSFYHDTDFLGEELDIVAAKSHEACQKLCTNAVRCQFFTYTPAQASCNEGKGKCYLKLSSNGSPTKILHGRGGISGYTLRLCKMDNECTTKIK |
NCBI | NP_000119 |
Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
Buffer/Preservatives | In 1x PBS, pH 7.4 |
Note | For research use only |
Expiration Date | 12 months from date of receipt. |
Western Blot detection against Immunogen (36.74 KDa).
Detection limit for recombinant GST tagged F11 is approximately 10 ng/ml as a capture antibody.
Immunoprecipitation of F11 transfected lysate using anti-F11 monoclonal antibody and Protein A Magnetic Bead, and immunoblotted with F11 MaxPab rabbit polyclonal antibody.
Western Blot analysis of F11 expression in transfected 293T cell line by F11 monoclonal antibody (M01), clone 2H8. Lane 1: F11 transfected lysate (70.1 KDa). Lane 2: Non-transfected lysate.