Enhancer of zeste homolog 2 Antibody / EZH2

SKU: orb1822916

Description

This gene encodes a member of the Polycomb-group (PcG) family. PcG family members form multimeric protein complexes, which are involved in maintaining the transcriptional repressive state of genes over successive cell generations. This protein associates with the embryonic ectoderm development protein, the VAV1 oncoprotein, and the X-linked nuclear protein. This protein may play a role in the hematopoietic and central nervous systems. Multiple alternatively splcied transcript variants encoding distinct isoforms have been identified for this gene.

Images & Validation

• Tested Applications: IHC-P, WB
• Dilution Range: Western blot: 1-2ug/ml,Immunohistochemistry (FFPE): 1-2ug/ml for 30 min at RT
• Reactivity: Human
• Application Notes:
  Optimal dilution of the EZH2 antibody should be determined by the researcher.

Key Properties

• Antibody Type: Primary Antibody
• Host: Mouse
• Clonality: Monoclonal
• Isotype: Mouse IgG, kappa
• Clone No.: EZH2/6988
• Immunogen: A recombinant partial protein sequence (within amino acids 300-550) from the human protein was used as the immunogen for the Enhancer of zeste homolog 2 antibody.
• Purification: Protein A/G affinity
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: 1 mg/ml in 1X PBS; rAlbumin free, sodium azide free
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

IHC staining of FFPE human bladder carcinoma with Enhancer of zeste homolog 2 antibody (clone EZH2/6988). HIER: boil tissue sections in pH9 10 mM Tris with 1 mM EDTA for 20 min and allow to cool before testing.

IHC staining of FFPE human Lynch syndrome colon carcinoma tissue with Enhancer of zeste homolog 2 antibody (clone EZH2/6988). HIER: boil tissue sections in pH9 10 mM Tris with 1 mM EDTA for 20 min and allow to cool before testing.

IHC staining of FFPE human bladder carcinoma with Enhancer of zeste homolog 2 antibody (clone EZH2/6988). HIER: boil tissue sections in pH9 10 mM Tris with 1 mM EDTA for 20 min and allow to cool before testing.

Western blot testing of human Jurkat cell lysate with Enhancer of zeste homolog 2 antibody (clone EZH2/6988). Expected molecular weight: 85-95 kDa.

Quick Database Links

UniProt

Q15910