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EYA4 Antibody (C-term)
Catalog Number: orb1927974
| Catalog Number | orb1927974 |
|---|---|
| Category | Antibodies |
| Description | Affinity Purified Rabbit Polyclonal Antibody (Pab) |
| Species/Host | Rabbit |
| Clonality | Polyclonal |
| Clone Number | RB23878 |
| Tested applications | FC, IHC-P, WB |
| Reactivity | Human |
| Isotype | Rabbit IgG |
| Antibody Type | Primary Antibody |
| Dilution range | WB: 1:1000, IHC-P: 1:50~100, FC: 1:10~50 |
| Form/Appearance | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification. |
| Conjugation | Unconjugated |
| MW | 69505 Da |
| Target | This EYA4 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 1-30 amino acids from the C-terminal region of human EYA4. |
| UniProt ID | O95677 |
| NCBI | NP_004091.3, NP_742101.2, NP_742103.1 |
| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles |
| Alternative names | Eyes absent homolog 4, EYA4 Read more... |
| Note | For research use only |
| Expiration Date | 12 months from date of receipt. |
Western blot analysis of EYA4 Antibody (C-term) in A2058 cell line lysates (35 ug/lane). EYA4 (arrow) was detected using the purified Pab.
EYA4 Antibody (C-term) flow cytometry analysis of HL-60 cells (bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
Formalin-fixed and paraffin-embedded human skeletal muscle reacted with EYA4 Antibody (C-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
