EYA2 Antibody

• Catalog Number: orb1526175
• Category: Antibodies
• Description: EYA2 Antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: IHC, IP
• Reactivity: Human
• Immunogen: between 1 and 50
• Concentration: 1000 µg/ml
• Dilution range: WB - Not recommended. Use rabbit anti-EYA2 antibody A302-688A.; IP - 2 - 5 µg/mg lysate; IHC - 1:500 - 1:2,000. Epitope retrieval with citrate buffer pH 6.0 is recommended for FFPE tissue sections.
• Form/Appearance: Whole IgG
• Conjugation: Unconjugated
• Target: EYA2
• UniProt ID: O00167
• NCBI: NP_005235.3
• Storage: 2 - 8°C
• Buffer/Preservatives: Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09% Sodium Azide
• Alternative names: eyes absent homolog 2; EAB1
• Note: For research use only
• Application notes: Format: Whole IgG
• Expiration Date: 12 months from date of receipt.

Detection of human EYA2 by western blot of immunoprecipitates. Samples: Whole cell lysate (1 mg for IP, 20% of IP loaded) from HEK293T cells. Antibodies: Affinity purified rabbit anti-EYA2 antibody orb1526175 used for IP at 3 µg/mg lysate.

Detection of human EYA2 by immunohistochemistry.Sample: FFPE section of human breast carcinoma. Antibody: Affinity purified rabbit anti-EYA2 (Cat. No. orb1526175) used at a dilution of 1: 1, 000 (1µg/ml). Detection: DAB