ExoN Antibody

• Catalog Number: orb1239077
• Category: Antibodies
• Description: ExoN Antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: ELISA, IHC
• Reactivity: Virus
• Isotype: IgG
• Immunogen: Anti-SARS-CoV-2 (COVID-19) NSP14 (ExoN) antibody (orb1239077) was raised against a peptide corresponding to 14 amino acids near the center of SARS-CoV-2 (COVID-19) NSP14 (ExoN) protein. The immunogen is located between 120-170 amino acids of SARS-CoV-2 (COVID-19) NSP14 (ExoN).
• Concentration: 1 mg/mL
• Dilution range: IHC: 0.5 μg/mL .Antibody validated: Immunohistochemistry in human samples. SARS-CoV-2 (COVID-19) NSP14 (ExoN) antibody can detect 2 ng of free peptide at 1 μg/mL in ELISA. All other applications and species not yet tested
• Form/Appearance: Liquid
• Conjugation: Unconjugated
• Target: ExoN
• UniProt ID: P0DTC1
• NCBI: YP_009725309.1
• Storage: SARS-CoV-2°COVID-19) NSP14 (ExoN) antibody can be stored at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
• Buffer/Preservatives: SARS-CoV-2 (COVID-19) NSP14 (ExoN) Antibody is supplied in PBS containing 0.02% sodium azide.
• Alternative names: Proofreading exoribonuclease, Guanine-N7 methyltra
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

Immunohistochemistry Validation of SARS-CoV-2 (COVID-19) NSP14 in COVID-19 Patient Lung. Immunohistochemical analysis of paraffin-embedded COVID-19 patient lung tissue using anti-SARS-CoV-2 (COVID-19) NSP14 antibody (orb1239077, 0.5 µg/mL). Tissue was fixed with formaldehyde and blocked with 10% serum for 1 h at RT; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody overnight at 4 ˚C. A goat anti-rabbit IgG H&L (HRP) at 1/250 was used as secondary. Counter stained with Hematoxylin. Strong signal of SARS-COV-2 NSP14 protein was observed in macrophage of COVID-19 patient lung, but not in non-COVID-19 patient lung.

ELISA Validation. Antibodies: SARS-CoV-2 (COVID-19) NSP14 (ExoN) Antibody, orb1239077. A direct ELISA was performed using immunogen peptide (orb1231678) as coating antigen and the anti-SARS-CoV-2 (COVID-19) NSP14 (ExoN) antibody as the capture antibody. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:20000 dilution. Detection range is from 0.3 ng/mL to 1000 ng/mL.