You have no items in your shopping cart.
You have no items in your shopping cart.
Catalog Number | orb1524400 |
---|---|
Category | Antibodies |
Description | ETV6 Antibody |
Species/Host | Rabbit |
Clonality | Polyclonal |
Tested applications | IHC, IP, WB |
Reactivity | Human |
Immunogen | Between 175 and 225 |
Concentration | 1000 µg/ml |
Dilution range | WB - 1:1,000 - 1:5,000; IP - 2 - 10 µg/mg lysate; IHC - 1:500 - 1:2,000. Epitope retrieval with citrate buffer pH 6.0 is recommended for FFPE tissue sections. |
Form/Appearance | Whole IgG |
Conjugation | Unconjugated |
Target | ETV6 |
UniProt ID | P41212 |
NCBI | NP_001978.1 |
Storage | 2 - 8°C |
Buffer/Preservatives | Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09% Sodium Azide |
Alternative names | ets variant gene 6 (TEL oncogene); TEL1 oncogene; Read more... |
Note | For research use only |
Application notes | Format: Whole IgG |
Expiration Date | 12 months from date of receipt. |
Detection of human ETV6 by western blot and immunoprecipitation. Samples: Whole cell lysate from Jurkat (50 µg for WB; 1 mg for IP, 20% of IP loaded) , HEK293T (T; 50 µg) and HeLa (H; 50 µg) cells. Antibodies: Affinity purified rabbit anti-ETV6 antibody orb1524400 used for WB at 0.4 µg/ml (A) and 1 µg/ml (B) and used for IP at 6 µg/mg lysate. ETV6 was also immunoprecipitated by rabbit anti-ETV6 antibody, which recognizes a downstream epitope. Detection: Chemiluminescence with exposure times of 3 minutes (A) and 30 seconds (B).
Detection of human ETV6 by immunohistochemistry.Sample: FFPE section of human colon carcinoma. Antibody: Affinity purified rabbit anti-ETV6 (Cat. No. orb1524400) used at a dilution of 1: 1, 000 (1µg/ml). Detection: DAB
ELISA, IHC, WB | |
Human, Mouse, Rat | |
Rabbit | |
Polyclonal | |
Unconjugated |
ELISA, IF, IHC-P, WB | |
Human, Mouse, Rat | |
Rabbit | |
Polyclonal | |
Unconjugated |
Filter by Rating