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Catalog Number | orb1477062 |
---|---|
Category | Proteins |
Description | Recombinant Staphylococcus aureus Type VII secretion system extracellular protein A(esxA) |
Tag | N-terminal 6xHis-SUMO-tagged |
Form/Appearance | Liquid or Lyophilized powder |
Buffer/Preservatives | If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0. |
Purity | Greater than 90% as determined by SDS-PAGE. |
Protein Sequence | MAMIKMSPEEIRAKSQSYGQGSDQIRQILSDLTRAQGEIAANWEGQAFSRFEEQFQQLSPKVEKFAQLLEEIKQQLNSTADAVQEQDQQLSNNFGLQ |
Protein Length | Full Length |
UniProt ID | Q6GCJ0 |
MW | 24.0 kDa |
Application notes | We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference. |
Endotoxins | Not test. |
Source | E.coli |
Biological Origin | Staphylococcus aureus (strain MSSA476) |
Expression Region | 1-97aa |
Storage | The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20℃/-80℃. The shelf life of lyophilized form is 12 months at -20℃/-80℃. |
Alternative names | (Ess extracellular protein A) |
Note | For research use only |
(Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.
Greater than 90% as determined by SDS-PAGE. | |
27 kDa | |
E.coli |
Greater than 90% as determined by SDS-PAGE. | |
11.3 kDa | |
Yeast |
Greater than 85% as determined by SDS-PAGE. | |
17.2 kDa | |
E.coli |
ELISA, ICC, IF, IHC-Fr, IHC-P, WB | |
Bacteria | |
Rabbit | |
Polyclonal | |
Unconjugated |