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Catalog Number | orb215946 |
---|---|
Category | Antibodies |
Description | Estrogen Receptor/ESR1 Antibody |
Species/Host | Rabbit |
Clonality | Polyclonal |
Tested applications | IHC, WB |
Reactivity | Human |
Isotype | Rabbit IgG |
Immunogen | E.coli-derived human Estrogen Receptor recombinant protein (Position: F425-V595). Human Estrogen Receptor shares 89% and 88% amino acid (aa) sequences identity with mouse and rat Estrogen Receptor, respectively. |
Concentration | Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml. |
Dilution range | Immunohistochemistry (Paraffin-embedded Section), 0.5-1μg/ml, Human, By HeatWestern blot, 0.1-0.5μg/ml, Human |
Form/Appearance | Lyophilized |
Conjugation | Unconjugated |
MW | 66216 MW |
UniProt ID | P03372 |
Storage | Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles. |
Alternative names | Estrogen receptor;ER;ER-alpha;Estradiol receptor;N Read more... |
Note | For research use only |
Application notes | WB: The detection limit for Estrogen Receptor is approximately 0.2ng/lane under reducing conditions. Tested Species: In-house tested species with positive results. By Heat: Boiling the paraffin sections in 10mM citrate buffer, pH6.0, for 20mins is required for the staining of formalin/paraffin sections. Other applications have not been tested. Optimal dilutions should be determined by end users. . Add 0.2ml of distilled water will yield a concentration of 500ug/ml. |
Expiration Date | 12 months from date of receipt. |
IHC-P image of human mammary cancer tissue using Estrogen Recepter beta 1 antibody
Western blot analysis of recombinant human ER protein at 0.5ng using Estrogen Recepter beta 1 antibody
Western blot analysis of MCF-7 Whole Cell Lysate at 40ug using Estrogen Recepter beta 1 antibody
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