Estrogen Receptor antibody

• Catalog Number: orb108879
• Category: Antibodies
• Description: Mouse monoclonal to Estrogen Receptor
• Species/Host: Mouse
• Clonality: Monoclonal
• Clone Number: TE111.5D11
• Tested applications: IP, WB
• Reactivity: Bovine, Canine, Hamster, Human, Porcine, Rabbit, Rat, Sheep
• Isotype: IgG1
• Concentration: See vial for concentration
• Form/Appearance: Provided as solution in phosphate buffered saline with 0.08% sodium azide
• Conjugation: Unconjugated
• Hazard Information: This product is intended FOR RESEARCH USE ONLY, and FOR TESTS IN VITRO, not for use in diagnostic or therapeutic procedures involving humans or animals.
• UniProt ID: P11474
• Source: Hybridoma produced by the fusion of splenocytes from mice immunized with the 35 kDa C-terminal fragment (aa 302-595) of human ER expressed in E. coli and mouse myeloma cells.
• Storage: Product should be stored at -20°C. Aliquot to avoid freeze/thaw cycles
• Buffer/Preservatives: Provided as solution in phosphate buffered saline with 0.08% sodium azide
• Note: For research use only
• Application notes: This antibody can be used gel supershift assays (neat concentration), immunoprecipitation on native and denatured lysate (2 µg/mg of protein lysate), Western blotting (1-2 µg/ml) and chromatin precipitation. Can also be used to block binding of estrogen to the estrogen receptor. Optimal concentration should be evaluated by serial dilutions.
• Expiration Date: 12 months from date of receipt.

Western blot analysis of human cells using Estrogen Receptor antibody