Estrogen Receptor alpha Antibody

• Catalog Number: orb534134
• Category: Antibodies
• Description: Estrogen receptor (ER) is an important regulator of growth and differentiation in the mammary gland. ER presence in breast tumors indicates an increased likelihood of response to anti-estrogen therapy.
• Species/Host: Mouse
• Clonality: Monoclonal
• Clone Number: NR3Ga-2
• Tested applications: IHC, WB
• Reactivity: Human
• Isotype: Mouse IgG2a, kappa
• Immunogen: Full length human protein was used as the immunogen for this ER alpha antibody.
• Antibody Type: Primary Antibody
• Dilution range: Western blot: 1-2ug/ml,Immunohistochemistry (FFPE): 1-2ug/ml for 30 min at RT
• Purity: Protein G affinity chromatography
• Conjugation: Unconjugated
• Formula: 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide
• Hazard Information: This ER alpha antibody is available for research use only.
• UniProt ID: P03372
• Storage: Store the ER alpha antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide).
• Buffer/Preservatives: 0.2 mg/ml in 1X PBS with 0.1 mg/ml rAlbumin (US sourced) and 0.05% sodium azide
• Note: For research use only
• Application notes: The optimal dilution of the ER alpha antibody for each application should be determined by the researcher.
• Expiration Date: 12 months from date of receipt.

Western blot testing of human MCF-7 lysate with Estrogen Receptor alpha antibody (clone NR3Ga-2). Expected molecular weight ~67 kDa.

IHC testing of human breast carcinoma stained with ER alpha antibody (clone NR3Ga-2). Staining of formalin-fixed tissues requires boiling tissue sections in pH9 10mM Tris with 1mM EDTA for 10-20 min followed by cooling at RT for 20 minutes.