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Estrogen Receptor alpha Antibody

Catalog Number: orb2642848

DispatchUsually dispatched within 5-10 working days
$ 780.00
Catalog Numberorb2642848
CategoryAntibodies
DescriptionThis monoclonal antibody is specific to estrogen receptor alpha (ER alpha) and shows minimal cross-reaction with other members of the family. ER is an important regulator of growth and differentiation in the mammary gland. Presence of ER in breast tumors indicates an increased likelihood of response to anti-estrogen (e.g. tamoxifen) therapy. It strongly stains nuclei of epithelial cells in breast carcinomas.
Species/HostMouse
ClonalityMonoclonal
Clone NumberESR1/3557
Tested applicationsFACS, IF, IHC-P
ReactivityHuman
IsotypeMouse IgG1, kappa
ImmunogenA recombinant human ESR1 protein fragment within amino acids 129-312 was used as the immunogen for the Estrogen Receptor alpha antibody.
Antibody TypePrimary Antibody
Dilution rangeImmunofluorescence: 1-2ug/ml,Flow cytometry: 1-2ug/million cells,Immunohistochemistry (FFPE): 1-2ug/ml for 30 min at RT
PurityProtein G affinity chromatography
ConjugationUnconjugated
Formula0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide
Hazard InformationThis Estrogen Receptor alpha antibody is available for research use only.
UniProt IDP03372
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Buffer/Preservatives0.2 mg/ml in 1X PBS with 0.1 mg/ml rAlbumin (US sourced) and 0.05% sodium azide
NoteFor research use only
Application notesOptimal dilution of the Estrogen Receptor alpha antibody should be determined by the researcher.1. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.
Expiration Date12 months from date of receipt.
Estrogen Receptor alpha Antibody

Immunofluorescence staining of human MCF-7 cells with Estrogen Receptor alpha antibody (green, clone ESR1/3557) and Phalloidin (red).

Estrogen Receptor alpha Antibody

IHC staining of FFPE human breast carcinoma with Estrogen Receptor alpha antibody (clone ESR1/3557). HIER: boil tissue sections in pH9 10mM Tris with 1mM EDTA for 10-20 min and allow to cool before testing.

Estrogen Receptor alpha Antibody

IHC staining of FFPE human breast carcinoma with Estrogen Receptor alpha antibody (clone ESR1/3557). HIER: boil tissue sections in pH9 10mM Tris with 1mM EDTA for 10-20 min and allow to cool before testing.

Estrogen Receptor alpha Antibody

Flow cytometry testing of fixed and permeabilized human MCF-7 cells with Estrogen Receptor alpha antibody (clone ESR1/3557); Red=isotype control, Blue= Estrogen Receptor alpha antibody.

Estrogen Receptor alpha Antibody

Analysis of HuProt (TM) microarray containing more than 19000 full-length human proteins using Estrogen Receptor alpha antibody (clone ESR1/3557). These results demonstrate the foremost specificity of the ESR1/3557 mAb. Z- and S- score: The Z-score represents the strength of a signal that an antibody (in combination with a fluorescently-tagged anti-IgG secondary Ab) produces when binding to a particular protein on the HuProt (TM) array. Z-scores are described in units of standard deviations (SD's) above the mean value of all signals generated on that array. If the targets on the HuProt (TM) are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD's) between the Z-scores. The S-score therefore represents the relative target specificity of an Ab to its intended target.

Estrogen Receptor alpha Antibody

SDS-PAGE analysis of purified, BSA-free Estrogen Receptor alpha antibody (clone ESR1/3557) as confirmation of integrity and purity.

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