Estrogen R antibody

• Catalog Number: orb241921
• Category: Antibodies
• Description: Estrogen R antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: ChIP, ELISA, IHC, WB
• Reactivity: Human, Rat
• Isotype: IgG
• Immunogen: Recombinant Human Estrogen receptor protein (10-591AA)
• Dilution range: WB:1:1000-1:5000, IHC-P:1:20-1:200
• Form/Appearance: Liquid
• Conjugation: Unconjugated
• Target: ESR1
• UniProt ID: P03372
• Storage: Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
• Buffer/Preservatives: Preservative: 0.03% Proclin 300. Constituents: 50% Glycerol
• Alternative names: anti ER-alpha antibody, anti Estradiol receptor an
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

Chromatin Immunoprecipitation MCF-7 (1.1*106) were cross-linked with formaldehyde, sonicated, and immunoprecipitated with 4ug anti-ESR1 or a control normal rabbit IgG. The resulting ChIP DNA was quantified using real-time PCR with primers again

Western blot aanalysis of Hela whole cell lysate (Lane1), MCF-7 whole cell lysate (Lane2), Rat brain tissue (Lane3), Colo320 whole cell lysate (Lane4)using Estrogen R antibody

Immunohistochemistry of paraffin-embedded human breast cancer tissue using Estrogen R antibody