Esrp-1/2 antibody

• Catalog Number: orb345206
• Category: Antibodies
• Description: Esrp-1/2 antibody
• Species/Host: Mouse
• Clonality: Monoclonal
• Clone Number: 23A7.C9
• Tested applications: ELISA, WB
• Reactivity: Mouse
• Isotype: IgG1
• Immunogen: Anti-Esrp-1/2 was produced by repeated immunizations of full length recombinant mouse Esrp-1 fusion protein.
• Concentration: 1.0 mg/mL
• Dilution range: ELISA: 1:750,000, WB: 1:1000
• Form/Appearance: Liquid (sterile filtered)
• Purity: This antibody was purified from tissue culture supernatant by Protein-A chromatography followed by extensive dialysis against the buffer stated above. This antibody reacts with both mouse Esrp-1 and Esrp-2 proteins. A BLAST analysis of the immunizing protein sequence shows 100% homology with Esrp-1 from mouse and a 91% sequence homology with Esrp-1 from human, pig, rat, opossum, horse, cattle, panda, dog, and chimpanzee. The binding epitope of this monoclonal antibody has not been mapped.
• Conjugation: Unconjugated
• UniProt ID: Q8K0G8
• NCBI: NP_789808.1
• Storage: Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
• Buffer/Preservatives: 0.01% (w/v) Sodium Azide
• Alternative names: mouse anti-Esrp1/2 antibody, mouse anti-Esrp1 anti
• Note: For research use only
• Application notes: This protein-A purified antibody has been tested for use western blotting. Specific conditions for reactivity should be optimized by the end user. Expect a band approximately 75.5 kDa in size corresponding to Esrp-1 and 77.4 kDa in size corresponding to Esrp-2 by western blotting in the appropriate cell lysate or extract.
• Expiration Date: 12 months from date of receipt.

Western blot analysis of 293T cell extracts. Lane 1: GFP-transfected. Lane 2: Esrp-1 transfected (arrow). Lane 3: Esrp-2 transfected using Esrp-1/2 antibody