You have no items in your shopping cart.
You have no items in your shopping cart.
Catalog Number | orb1252088 |
---|---|
Category | Antibodies |
Description | ESR2 Antibody |
Species/Host | Mouse |
Clonality | Monoclonal |
Clone Number | NR3Gb-1 |
Tested applications | FC, IF, IHC, WB |
Reactivity | Equine, Human, Monkey, Mouse, Porcine, Rat, Sheep |
Isotype | IgG2a |
Immunogen | The C-terminus fragment of recombinant human Estrogen Receptor beta protein was used as the immunogen for this antibody. |
Concentration | 0.2 mg/mL |
Dilution range | Flow Cytometry: 0.5-1ug/10e6 cellsIF: 1-2 ug/mlWB: 0.5-1 ug/mlIHC (FFPE): 0.5-1 ug/ml for 30 minutes at RT (1)Prediluted format : incubate for 30 min at RT (2)The concentration stated for each application is a general starting point. Variations in protocols, secondaries and substrates may require the Estrogen Receptor beta antibody to be titered up or down for optimal performance.1. Staining of formalin-fixed tissues requires boiling tissue sections in 10mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes.2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min. |
Form/Appearance | Liquid |
Conjugation | Unconjugated |
Target | ESR2 |
UniProt ID | Q92731 |
Storage | Aliquot and Store at 2-8°C. Avoid freez-thaw cycles. |
Buffer/Preservatives | PBS with 0.1 mg/ml rAlbumin and 0.05% sodium azide |
Alternative names | Estrogen receptor beta, ER-beta, Nuclear receptor Read more... |
Note | For research use only |
Application notes | Flow Cytometry: 0.5-1ug/10e6 cellsIF: 1-2 ug/mlWB: 0.5-1 ug/mlIHC (FFPE): 0.5-1 ug/ml for 30 minutes at RT (1)Prediluted format : incubate for 30 min at RT (2)The concentration stated for each application is a general starting point. Variations in protocols, secondaries and substrates may require the Estrogen Receptor beta antibody to be titered up or down for optimal performance.1. Staining of formalin-fixed tissues requires boiling tissue sections in 10mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes.2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min. |
Expiration Date | 12 months from date of receipt. |
IHC testing of ovarian cancer stained with Estrogen Receptor beta antibody.
IHC testing of gastric carcinoma stained with Estrogen Receptor beta antibody.
FACS testing of MCF-7 cells: Black = cells alone; Green = isotype control; Red = Estrogen Receptor beta antibody PE conjugate.
ICC, IF, IHC-P, WB | |
Human, Mouse, Rat | |
Rabbit | |
Polyclonal | |
Unconjugated |
FC, IF, IHC, WB | |
Equine, Human, Monkey, Mouse, Porcine, Rat, Sheep | |
Mouse | |
Monoclonal | |
Unconjugated |
FC, IF, IHC-P, WB | |
Equine, Human, Monkey, Mouse, Porcine, Rat, Sheep | |
Mouse | |
Monoclonal | |
Unconjugated |
FC, IF, IHC-P, WB | |
Human | |
Mouse | |
Monoclonal | |
Unconjugated |
ELISA, ICC, IHC-Fr, IHC-P, WB | |
Human, Mouse, Rat | |
Rabbit | |
Polyclonal | |
Unconjugated |
Filter by Rating