Erythropoietin Antibody / EPO

• Catalog Number: orb2640182
• Category: Antibodies
• Description: Erythropoietin / EPO is a secreted, glycosylated cytokine hormone composed of four alpha helical bundles. It is the primary factor responsible for regulating erythropoiesis during steady-state conditions and in response to blood loss and hemorrhage in the adult organism. Erythropoietin is synthesized by the kidney and stimulates the proliferation and maturation of bone marrow erythroid precursor cells. The protein is found in the plasma and regulates red cell production by promoting erythroid differentiation and initiating hemoglobin synthesis.
• Species/Host: Mouse
• Clonality: Monoclonal
• Clone Number: EPO/1368
• Tested applications: ELISA, IHC-P, WB
• Reactivity: Human
• Isotype: Mouse IgG1, kappa
• Immunogen: A human recombinant partial protein was used as the immunogen for the EPO antibody.
• Antibody Type: Primary Antibody
• Dilution range: ELISA: 2-4ug/ml (order BSA/azide-free format),Western blot: 1-2ug/ml,Immunohistochemistry (FFPE): 2-4ug/ml for 30 min at RT
• Purity: Protein G affinity chromatography
• Conjugation: Unconjugated
• Formula: 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide
• Hazard Information: This EPO antibody is available for research use only.
• UniProt ID: P01588
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: 0.2 mg/ml in 1X PBS with 0.1 mg/ml rAlbumin (US sourced) and 0.05% sodium azide
• Note: For research use only
• Application notes: Titering of the EPO antibody may be required for optimal performance.1. Required HIER: boil tissue sections in 10mM citrate buffer, pH 6, for 10-20 min.
• Expiration Date: 12 months from date of receipt.

Western blot testing of with EPO antibody (clone EPO/1368). Predicted molecular weight: 18-34 kDa depending on glycosylation level.

SDS-PAGE Analysis of Purified, BSA-Free EPO Antibody (clone EPO/1368). Confirmation of Integrity and Purity of the Antibody.

IHC staining of FFPE human renal cell carcinoma with EPO antibody. HIER: boil tissue sections in pH6, 10mM citrate buffer, for 10-20 min followed by cooling at RT for 20 min.

Analysis of HuProt (TM) microarray containing more than 19, 000 full-length human proteins using EPO antibody (clone EPO/1368). These results demonstrate the foremost specificity of the EPO/1368 mAb. Z- and S- score: The Z-score represents the strength of a signal that an antibody (in combination with a fluorescently-tagged anti-IgG secondary Ab) produces when binding to a particular protein on the HuProt (TM) array. Z-scores are described in units of standard deviations (SD's) above the mean value of all signals generated on that array. If the targets on the HuProt (TM) are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD's) between the Z-scores. The S-score therefore represents the relative target specificity of an Ab to its intended target.