ERN1 monoclonal antibody (M02), clone 8D12

• Catalog Number: orb2293180
• Category: Antibodies
• Description: Mouse monoclonal antibody raised against a partial recombinant ERN1.
• Clonality: Monoclonal
• Species/Host: Mouse
• Isotype: IgG2a Kappa
• Conjugation: Unconjugated
• Reactivity: Human
• Buffer/Preservatives: In 1x PBS, pH 7.4
• Immunogen: ERN1 (AAC25991, 401 a.a. ~ 500 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
• Protein Sequence: EEVINLVDQTSENAPTTVSRDVEEKPAHAPARPEAPVDSMLKDMATIILSTFLLIGWVAFIITYPLSMHQQQQLQHQQFQKELEKIQLLQQQQQQLPFHP
• Tested applications: ELISA, IF, PLA, WB
• Clone Number: 8D12
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Note: For research use only
• NCBI: AAC25991

Detection limit for recombinant GST tagged ERN1 is approximately 0.03 ng/ml as a capture antibody.

ERN1 monoclonal antibody (M02), clone 8D12. Western Blot analysis of ERN1 expression in LNCaP.

Immunofluorescence of monoclonal antibody to ERN1 on HeLa cell. [antibody concentration 25 ug/ml]

Proximity Ligation Analysis of protein-protein interactions between TRAF2 and ERN1. HeLa cells were stained with anti-TRAF2 rabbit purified polyclonal 1:1200 and anti-ERN1 mouse monoclonal antibody 1:50. Each red dot represents the detection of protein-protein interaction complex, and nuclei were counterstained with DAPI (blue).

Western Blot detection against Immunogen (36.63 KDa).