ERK1/2, Phospho (T202/Y204, T185/Y187) Antibody

• Catalog Number: orb1524514
• Category: Antibodies
• Description: ERK1/2, Phospho (T202/Y204, T185/Y187) Antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: WB
• Reactivity: Human
• Immunogen: surrounding threonine 185 and tyrosine 187
• Concentration: 1000 µg/ml
• Dilution range: WB - 1:1,000 - 1:5,000; IP - Not recommended
• Form/Appearance: Whole IgG
• Conjugation: Unconjugated
• Target: ERK1/2, Phospho (T202/Y204, T185/Y187)
• UniProt ID: P28482, P27361
• NCBI: NP_002737.2
• Storage: 2 - 8°C
• Buffer/Preservatives: Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09% Sodium Azide
• Alternative names: PRKM1; p38; p40; p41; p41mapk; P42MAPK; ERK2; PRKM
• Note: For research use only
• Application notes: Format: Whole IgG
• Expiration Date: 12 months from date of receipt.

Detection of human phospho-ERK1 and ERK2 by western blot (WB). Samples: Whole cell lysate (50 µg for WB; 1 mg for IP, 20% of IP loaded) from HeLa cells that were mock treated (-) or treated with TPA (+). Antibodies: Affinity purified rabbit anti-Phospho ERK1/2 orb1524514 used for WB at 0.1 µg/ml (A) and 1 µg/ml (B). In (B) , ERK1 was immunoprecipitated with affinity purified rabbit anti-ERK1 antibody. To demonstrate the presence of total ERK1, blots (A) and (B) were stripped and re-probed with anti-ERK1 antibody. Detection: Chemiluminescence with exposure times of 30 seconds (A) and (B).