ERK1 (phospho-Thr183/185) antibody

• Catalog Number: orb10606
• Category: Tools
• Description: ERK1 (phospho-Thr183/185) antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: ICC, IF, IHC-P
• Predicted Reactivity: Bovine, Canine, Equine, Gallus, Guinea pig, Rabbit, Rat
• Reactivity: Human, Mouse
• Isotype: IgG
• Immunogen: KLH conjugated Synthesised phosphopeptide derived from mouse ERK1 around the phosphorylation site of Thr183/Tyr185 (FL(p-T)E(p-Y)V)
• Concentration: 1mg/ml
• Dilution range: WB=1:500-2000, IHC-P=1:100-500, ICC=1:100, IF=1:100-500, IHC-F=1:100-500, ELISA=1:5000-10000
• Form/Appearance: Liquid
• Conjugation: Unconjugated
• MW: 42/44 kDa
• Target: phospho-ERK1 + 2 (Thr183/Tyr185)
• UniProt ID: Q63844
• Storage: Shipped at 4°C. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.
• Buffer/Preservatives: 0.01M TBS(pH7.4) with 1% rAlbumin, 0.03% Proclin300 and 50% Glycerol.
• Alternative names: ERK1 + ERK2 (phospho Thr183/Tyr185); phospho-ERK1/
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

References

Zhao, Haiyan et al. Inhibition of endocan attenuates monocrotaline-induced connective tissue disease related pulmonary arterial hypertension Int Immunopharmacol, 42, 115-121 (2017)

PubMed: 27912147

Immunohistochemical staining of human lung cancer tissue using ERK1 (phospho-Thr183-185) antibody.

Flow cytometric analysis of unstained cells using ERK1 (phospho-Thr183-185) antibody.

Western blot analysis of mouse lung tissue (Lane 1), mouse kidney lysates (Lane 2) using ERK1 (phospho-Thr183-185) antibody.

Flow cytometric analysis of U251 cell using ERK1 (phospho-Thr183-185) antibody.