ERCC4 Antibody

• Catalog Number: orb1255985
• Category: Antibodies
• Description: ERCC4 Antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: IP, WB
• Reactivity: Human, Mouse, Rat
• Isotype: IgG
• Immunogen: A synthetic peptide corresponding to a sequence within amino acids 600-700 of human ERCC4 (NP_005227.1).
• Concentration: batch dependent
• Dilution range: WB: 1:500 - 1:2000IP: 1:20 - 1:50
• Form/Appearance: Liquid
• Conjugation: Unconjugated
• MW: Observed: 120kDa
• Target: ERCC4
• UniProt ID: Q92889
• Storage: Store at -20°C. Avoid freeze / thaw cycles.
• Buffer/Preservatives: PBS with 0.02% sodium azide, 50% glycerol, pH 7.3.
• Alternative names: ERCC4, ERCC11, DNA repair endonuclease XPF, XPF
• Note: For research use only
• Application notes: WB: 1:500 - 1:2000IP: 1:20 - 1:50
• Expiration Date: 12 months from date of receipt.

Western blot analysis of extracts of various cell lines, using ERCC4 antibody (orb1255985) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25 ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.

Immunoprecipitation analysis of 150 ug extracts of 293T cells using 3 ug ERCC4 antibody (orb1255985). Western blot was performed from the immunoprecipitate using ERCC4 antibody (orb1255985) at a dilution of 1:1000.