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ERCC4 Antibody
Catalog Number: orb1255985
| Catalog Number | orb1255985 |
|---|---|
| Category | Antibodies |
| Description | ERCC4 Antibody |
| Species/Host | Rabbit |
| Clonality | Polyclonal |
| Tested applications | IP, WB |
| Reactivity | Human, Mouse, Rat |
| Isotype | IgG |
| Immunogen | A synthetic peptide corresponding to a sequence within amino acids 600-700 of human ERCC4 (NP_005227.1). |
| Antibody Type | Primary Antibody |
| Concentration | batch dependent |
| Form/Appearance | Liquid |
| Conjugation | Unconjugated |
| MW | Observed: 120kDa |
| Target | ERCC4 |
| UniProt ID | Q92889 |
| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
| Buffer/Preservatives | Liquid |
| Alternative names | ERCC4, ERCC11, DNA repair endonuclease XPF, XPF Read more... |
| Note | For research use only |
| Application notes | WB: 1:500 - 1:2000IP: 1:20 - 1:50 |
| Expiration Date | 12 months from date of receipt. |
Western blot analysis of extracts of various cell lines, using ERCC4 antibody (orb1255985) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25 ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.
Immunoprecipitation analysis of 150 ug extracts of 293T cells using 3 ug ERCC4 antibody (orb1255985). Western blot was performed from the immunoprecipitate using ERCC4 antibody (orb1255985) at a dilution of 1:1000.
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