ErbB3 Antibody

• Catalog Number: orb1523120
• Category: Antibodies
• Description: ErbB3 Antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: IP, WB
• Reactivity: Human
• Immunogen: Between 1292 and 1342
• Concentration: 1000 µg/ml
• Dilution range: WB - 1:1,000 - 1:5,000; IP - 2 - 10 µg/mg lysate
• Form/Appearance: Whole IgG
• Conjugation: Unconjugated
• Target: ErbB3
• UniProt ID: P21860
• NCBI: NP_001973.2
• Storage: 2 - 8°C
• Buffer/Preservatives: Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09% Sodium Azide
• Alternative names: v-erb-b2 avian erythroblastic leukemia viral oncog
• Note: For research use only
• Application notes: Format: Whole IgG
• Expiration Date: 12 months from date of receipt.

Detection of human ErbB3 by western blot. Samples: Whole cell lysate (50 µg) prepared using RIPA buffer from HeLa, HEK293T, Jurkat, and MCF-7 cells. Antibodies: Affinity purified rabbit anti-ErbB3 antibody orb1523120 used for WB at 0.4 µg/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.

Detection of human ErbB3 by western blot of immunoprecipitates. Samples: Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) prepared using RIPA buffer from MCF-7 cells. Antibodies: Affinity purified rabbit anti-ErbB3 antibody orb1523120 used for IP at 6 µg per reaction.