ERAB/HSD17B10 Antibody

• Catalog Number: orb251519
• Category: Antibodies
• Description: ERAB/HSD17B10 Antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: FC, ICC, IF, IHC, WB
• Reactivity: Human
• Isotype: Rabbit IgG
• Immunogen: E.coli-derived human ERAB recombinant protein (Position: E48-P261). Human ERAB shares 87% and 88% amino acid (aa) sequence identity with mouse and rat ERAB, respectively.
• Concentration: Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.
• Dilution range: Western blot, 0.1-0.5μg/ml Immunohistochemistry (Paraffin-embedded Section), 0.5-1μg/ml, By Heat Immunocytochemistry/Immunofluorescence, 2μg/ml Flow Cytometry, 1-3μg/1x106 cells
• Form/Appearance: Lyophilized
• Conjugation: Unconjugated
• MW: 26923 MW
• UniProt ID: Q99714
• Storage: Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
• Alternative names: 3-hydroxyacyl-CoA dehydrogenase type-2;1.1.1.35;17
• Note: For research use only
• Application notes: WB: The detection limit for ERAB is approximately 0.1ng/lane under reducing conditions. Tested Species: In-house tested species with positive results. By Heat: Boiling the paraffin sections in 10mM citrate buffer, pH6.0, for 20mins is required for the staining of formalin/paraffin sections. Other applications have not been tested. Optimal dilutions should be determined by end users. . Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
• Expiration Date: 12 months from date of receipt.

Flow Cytometry analysis of A549 cells using anti-ERAB antibody (Blue line).Isotype control antibody (Green line) was rabbit IgG .Unlabelled sample (Red line) was also used as a control.

Flow Cytometry analysis of A431 cells using anti-ERAB antibody (Blue line).Isotype control antibody (Green line) was rabbit IgG .Unlabelled sample (Red line) was also used as a control.

WB analysis of ERAB using anti-ERAB antibody.Lane 1:human Jurkat cell; 2:human 293T cell; 3:human HeLa cell; 4:human MOLT4 cell.

IF analysis of ERAB using anti-ERAB antibody. ERAB was detected in immunocytochemical section of U20S cells.

IHC analysis of ERAB using anti-ERAB antibody. ERAB was detected in paraffin-embedded section of Human Mammary Cancer Tissue.

IHC analysis of ERAB using anti-ERAB antibody.ERAB was detected in immunocytochemical section of MCF-7 cell.

IHC analysis of ERAB using anti-ERAB antibody.ERAB was detected in immunocytochemical section of MCF-7 cell.

IHC analysis of ERAB using anti-ERAB antibody.ERAB was detected in immunocytochemical section of HeLa cell.

IHC analysis of ERAB using anti-ERAB antibody.ERAB was detected in immunocytochemical section of SMMC-7721 cell.