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Catalog Number | orb1252225 |
---|---|
Category | Antibodies |
Description | EPO Antibody |
Species/Host | Mouse |
Clonality | Monoclonal |
Clone Number | EPO/1368 |
Tested applications | FC, IF, IHC-P, WB |
Reactivity | Human |
Isotype | IgG |
Immunogen | A human partial recombinant protein was used as the immunogen for the EPO antibody. |
Concentration | 0.2 mg/mL |
Dilution range | WB: 0.5-1 ug/mlIF: 1-2 ug/mlImmunohistochemistry (FFPE): 4-8 ug/ml for 30 min at RT (1)Flow Cytometry: 0.5-1 ug/million cells in 0.1mlTitering of the EPO antibody may be required for optimal performance.1. Required HIER: boil tissue sections in 10mM citrate buffer, pH 6, for 10-20 min. |
Form/Appearance | Liquid |
Conjugation | Unconjugated |
Target | EPO |
UniProt ID | P01588 |
Storage | Aliquot and Store at 2-8°C. Avoid freez-thaw cycles. |
Buffer/Preservatives | PBS with 0.1 mg/ml rAlbumin and 0.05% sodium azide |
Alternative names | Erythropoietin, Epoetin, EPO Read more... |
Note | For research use only |
Application notes | WB: 0.5-1 ug/mlIF: 1-2 ug/mlImmunohistochemistry (FFPE): 4-8 ug/ml for 30 min at RT (1)Flow Cytometry: 0.5-1 ug/million cells in 0.1mlTitering of the EPO antibody may be required for optimal performance.1. Required HIER: boil tissue sections in 10mM citrate buffer, pH 6, for 10-20 min. |
Expiration Date | 12 months from date of receipt. |
Western blot testing of with EPO antibody (clone EPO/1368). Predicted molecular weight: 18-34 kDa depending on glycosylation level.
SDS-PAGE Analysis of Purified, BSA-Free EPO Antibody (clone EPO/1368). Confirmation of Integrity and Purity of the Antibody.
ICC, IF, IHC, WB | |
Human, Mouse, Rat | |
Polyclonal | |
Unconjugated |
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