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Catalog Number | orb1956159 |
---|---|
Category | Proteins |
Description | EphA2 Protein, Human, Recombinant (His) is expressed in HEK293 mammalian cells with His tag. The predicted molecular weight is 57.6 kDa and the accession number is P29317. |
Tag | C-His |
Purity | ≥ 95 % as determined by SDS-PAGE. ≥ 90 % as determined by SEC-HPLC. |
MW | 57.6 kDa (predicted); 65-75 kDa (reducing conditions) |
UniProt ID | P29317 |
Expression System | HEK293 Cells |
Biological Origin | Human |
Biological Activity | EphA2 Protein, Human, Recombinant (His) is expressed in HEK293 mammalian cells with His tag. The predicted molecular weight is 57.6 kDa and the accession number is P29317. |
Expression Region | A DNA sequence encoding the human EPHA2 (P29317) (Met1-Asn534) was expressed with a polyhistidine tag at the C-terminus. Predicted N terminal: Ala 24 |
Storage | -20°C |
Note | For research use only |
Application notes | A Certificate of Analysis (CoA) containing reconstitution instructions is included with the products. Please refer to the CoA for detailed information. |
Expiration Date | 6 months from date of receipt. |
The purity of the protein is greater than 95% as determined by SDS-PAGE and Coomassie blue staining. | |
The protein has a predicted molecular mass of 57.0 kDa after removal of the signal peptide. The apparent molecular mass of EPHA2-His is approximately 55-70 kDa due to glycosylation. | |
Mammalian |
98.00% | |
72.1 kDa (predicted); 62 kDa (reducing conditions) |
98.00% | |
45 KDa (reducing condition) |
98.20% | |
59.4 kDa (predicted) |
> 97% by SDS-PAGE. | |
Recombinant Human EphA2 Protein is produced by HEK293 expression system. The target protein is expressed with sequence (Gln25-Asn534) of human EphA2 (Accession #NP_004422.2) fused with a 6��His Tag at the C-terminus. |