Eph receptor A2/EPHA2 Antibody

• Catalog Number: orb402207
• Category: Antibodies
• Description: Eph receptor A2/EPHA2 Antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: ELISA, FC, ICC, IF, WB
• Reactivity: Human, Mouse, Rat
• Isotype: Rabbit IgG
• Immunogen: E. coli-derived human Eph receptor A2 recombinant protein (Position: M851-N970).
• Concentration: Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.
• Dilution range: Western blot, 0.1-0.5μg/ml Immunocytochemistry/Immunofluorescence, 5μg/ml Flow Cytometry, 1-3μg/1x106 cells Direct ELISA, 0.1-0.5μg/ml
• Form/Appearance: Lyophilized
• Conjugation: Unconjugated
• MW: 125 kDa
• UniProt ID: P29317
• Storage: Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
• Alternative names: Ephrin type-A receptor 2; Epithelial cell kinase;
• Note: For research use only
• Application notes: Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
• Expiration Date: 12 months from date of receipt.

Flow Cytometry analysis of A549 cells using anti-Eph receptor A2 antibody (Blue line).Isotype control antibody (Green line) was rabbit IgG .Unlabelled sample (Red line) was also used as a control.

Flow Cytometry analysis of U20S cells using anti-Eph receptor A2 antibody (Blue line).Isotype control antibody (Green line) was rabbit IgG .Unlabelled sample (Red line) was also used as a control.

WB analysis of Eph receptor A2 using anti-Eph receptor A2 antibody.Lane 1:human HeLa Cell;2:human U-87MG Cell;3:human SHG-44 Cell;4:human COLO-320 Cell;5:human SK-OV-3 Cell;6:human A549 Cell;7:mouse HEPA1-6 Cell.

IF analysis of Eph receptor A2 using anti-Eph receptor A2 antibody. Eph receptor A2 was detected in immunocytochemical section of PC-3 cells.