EpCAM Antibody

• Catalog Number: orb2643514
• Category: Antibodies
• Description: EGP40 is a 40-43kDa transmembrane epithelial glycoprotein, also identified as epithelial specific antigen (ESA), or epithelial cellular adhesion molecule (Ep-CAM). It is expressed on baso-lateral cell surface in most simple epithelia and a vast majority of carcinomas. This antibody has been used to distinguish adenocarcinoma from pleural mesothelioma and hepatocellular carcinoma. This antibody is also useful in distinguishing serous carcinomas of the ovary from mesothelioma.
• Clonality: Monoclonal
• Species/Host: Mouse
• Isotype: Mouse IgG, kappa
• Conjugation: Unconjugated
• Reactivity: Canine, Feline, Human
• Buffer/Preservatives: 0.2 mg/ml with 0.1 mg/ml rAlbumin (US sourced), 0.05% sodium azide
• Purity: Protein G affinity chromatography
• Immunogen: A human partial recombinant protein corresponding to amino acids 100-224 (extracellular domain) was used as the immunogen for the EpCAM antibody.
• UniProt ID: P16422
• Tested applications: IF, IHC-P, WB
• Dilution range: Western blot: 1-2ug/ml,Immunofluorescence: 1-3ug/ml,Immunohistochemistry (FFPE): 1-2ug/ml
• Application notes: Optimal dilution of the antibody should be determined by the researcher.
• Antibody Type: Primary Antibody
• Clone Number: EGP40/1373
• Formula: 0.2 mg/ml with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Hazard Information: This EpCAM antibody is available for research use only.
• Note: For research use only

Immunofluorescent staining of human MCF7 cells with EpCAM antibody (clone EGP40/1373, green) and Reddot nuclear stain (red).

IHC staining of FFPE human fallopian tube with EpCAM antibody (clone EGP40/1373). HIER: boil tissue sections in pH9 10mM Tris with 1mM EDTA for 10-20 min and allow to cool before testing.

Western blot testing of human lung tissue lysate with EpCAM antibody. Expected molecular weight: ~35 kDa (unmodified), 40-43 kDa (glycosylated).

Analysis of HuProt (TM) microarray containing more than 19000 full-length human proteins using EpCAM antibody (clone EGP40/1373). These results demonstrate the foremost specificity of the EGP40/1373 mAb. Z- and S- score: The Z-score represents the strength of a signal that an antibody (in combination with a fluorescently-tagged anti-IgG secondary Ab) produces when binding to a particular protein on the HuProt (TM) array. Z-scores are described in units of standard deviations (SD's) above the mean value of all signals generated on that array. If the targets on the HuProt (TM) are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD's) between the Z-scores. The S-score therefore represents the relative target specificity of an Ab to its intended target.

SDS-PAGE analysis of purified, BSA-free EpCAM antibody (clone EGP40/1373) as confirmation of integrity and purity.

IHC staining of FFPE human rectal mass with EpCAM antibody (clone EGP40/1373). HIER: boil tissue sections in pH9 10mM Tris with 1mM EDTA for 10-20 min and allow to cool before testing.

IHC staining of FFPE dog colon with EpCAM antibody (clone EGP40/1373). HIER: boil tissue sections in pH9 10mM Tris with 1mM EDTA for 10-20 min and allow to cool before testing.

IHC staining of FFPE dog bladder with EpCAM antibody (clone EGP40/1373). HIER: boil tissue sections in pH9 10mM Tris with 1mM EDTA for 10-20 min and allow to cool before testing.

IHC staining of FFPE cat small intestine with EpCAM antibody (clone EGP40/1373). HIER: boil tissue sections in pH9 10mM Tris with 1mM EDTA for 10-20 min and allow to cool before testing.