EPB41L5 Antibody

• Catalog Number: orb1247574
• Category: Antibodies
• Description: EPB41L5 Antibody
• Species/Host: Goat
• Clonality: Polyclonal
• Tested applications: ELISA, WB
• Reactivity: Human
• Immunogen: The immunogen for this antibody is: C-ENLPQSPGTDQHD
• Concentration: 500 ug/mL
• Dilution range: Peptide ELISA: antibody detection limit dilution 1:8000.Western Blot:Approx 80kDa band observed in Human Brain (Hippocampus lysates (calculated MW of 81.9kDa according to NP_065960.2). Recommended concentration: 1-3ug/ml. An additional band of unknown identity was also consistently observed at 140kDa. This band was successfully blocked by incubation with the immunizing peptide. We would appreciate any feedback from people in the field - have any such results been reported with other antibodies/lysates? Have any further splice variants/modified forms been reported?
• Form/Appearance: Liquid
• Conjugation: Unconjugated
• Target: EPB41L5
• UniProt ID: Q9HCM4
• NCBI: NP_065960.2
• Storage: Aliquot and store at -20°C. Minimize freezing and thawing.
• Buffer/Preservatives: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
• Alternative names: EPB41L5, erythrocyte membrane protein band 4.1 lik
• Note: For research use only
• Application notes: Peptide ELISA: antibody detection limit dilution 1:8000.Western Blot:Approx 80kDa band observed in Human Brain (Hippocampus lysates (calculated MW of 81.9kDa according to NP_065960.2). Recommended concentration: 1-3ug/ml. An additional band of unknown identity was also consistently observed at 140kDa. This band was successfully blocked by incubation with the immunizing peptide. We would appreciate any feedback from people in the field - have any such results been reported with other antibodies/lysates? Have any further splice variants/modified forms been reported?
• Expiration Date: 12 months from date of receipt.

orb1247574 (1 ug/ml) staining of Human Brain (Hippocampus) lysate (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.