EP300 (phospho-S1834) antibody

• Catalog Number: orb215463
• Category: Antibodies
• Description: Rabbit polyclonal antibody to EP300
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: IH, WB
• Reactivity: Human, Mouse, Rat
• Immunogen: KLH-conjugated synthetic phosphopeptide corresponding to residues surrounding S1834 of human p300 protein. The exact sequence is proprietary.
• Dilution range: WB: 1-500-1-1000
• Form/Appearance: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
• Conjugation: Unconjugated
• Target: EP300
• Entrez: 328572, 2033
• UniProt ID: Q09472, B2RWS6
• Source: Rabbit
• Storage: Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.
• Buffer/Preservatives: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
• Alternative names: anti P300 antibody, anti Histone acetyltransferase
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

Western blot analysis of p300 (Phospho-S1834) expression in A549 (A), Raw264.7 (B), PC12 (C) whole cell lysates. (Predicted band size: 264 kD; Observed band size: 264 kD)

Immunohistochemical analysis of p300 (Phospho-S1834) staining in human breast cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (Phospho-H 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.