Enterokinase/EK Protein, Bovine, Recombinant

• Catalog Number: orb1955124
• Category: Proteins
• Description: Enterokinase is a member of the trypsin family of serine proteases. The precursor protein is cleaved into two chains which then forms a heterodimer linked by a disulfide bond. The heavy chain anchors enterokinase in the intestinal brush border membrane and the light chain is the catalytic subunit, which initiates conversion activation of a subset of pancreatic proteolytic proenzymes. Enterokinase is the physiological activator of trypsinogen and has a specificity for the sequence (Asp)4-Lys-Ile. The mature trypsin in turn activates other proenzymes including chymotrypsinogen, procarboxypeptidases, and proelastases. In addition, Enterokinase is a tool protease widely utilized in the cleavage of recombinant fusion proteins.
• Tag: Tag Free
• Purity: 95.00%
• MW: 26.1kDa (predicted); 45kDa (reducing condition, due to glycosylation)
• Expression System: P. pastoris (Yeast)
• Biological Origin: Bovine
• Biological Activity: Enterokinase is a member of the trypsin family of serine proteases. The precursor protein is cleaved into two chains which then forms a heterodimer linked by a disulfide bond. The heavy chain anchors enterokinase in the intestinal brush border membrane and the light chain is the catalytic subunit, which initiates conversion activation of a subset of pancreatic proteolytic proenzymes. Enterokinase is the physiological activator of trypsinogen and has a specificity for the sequence (Asp)4-Lys-Ile. The mature trypsin in turn activates other proenzymes including chymotrypsinogen, procarboxypeptidases, and proelastases. In addition, Enterokinase is a tool protease widely utilized in the cleavage of recombinant fusion proteins.
• Expression Region: A DNA sequence encoding the light chain (Ile 801-His 1035) of bovine Enterokinase (NP_776864) was expressed.
• Storage: -20°C
• Note: For research use only
• Application notes: Resuspend the enzyme powder with sterile water. Keep reconstituted enzyme at -20℃ in aliquots.
• Expiration Date: 6 months from date of receipt.