ENPP2/AUTOTAXIN Antibody

• Catalog Number: orb1248327
• Category: Antibodies
• Description: ENPP2/AUTOTAXIN Antibody
• Species/Host: Goat
• Clonality: Polyclonal
• Tested applications: ELISA, IHC, WB
• Reactivity: Human
• Immunogen: The immunogen for this antibody is: PDHLTSCVRPDVRVS
• Concentration: 500 ug/mL
• Dilution range: Peptide ELISA: antibody detection limit dilution 1:128000.Western Blot:Approx 100kDa band observed in Human Placenta lysates (calculated MW of 105kDa according to NP_006200.3). Recommended concentration: 0.3-1ug/ml. Primary incubation was 1 hour.Immunohistochemistry:Paraffin embedded Human Liver and Brain (Cortex). Recommended concentration: 5ug/ml.
• Form/Appearance: Liquid
• Conjugation: Unconjugated
• MW: Approx 100kDa
• Target: ENPP2 / AUTOTAXIN
• UniProt ID: Q13822
• NCBI: NP_001035181.1, NP_006200.3, NP_001124335.1
• Storage: Aliquot and store at -20°C. Minimize freezing and thawing.
• Buffer/Preservatives: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
• Alternative names: ENPP2, ectonucleotide pyrophosphatase/phosphodiest
• Note: For research use only
• Application notes: Peptide ELISA: antibody detection limit dilution 1:128000.Western Blot:Approx 100kDa band observed in Human Placenta lysates (calculated MW of 105kDa according to NP_006200.3). Recommended concentration: 0.3-1ug/ml. Primary incubation was 1 hour.Immunohistochemistry:Paraffin embedded Human Liver and Brain (Cortex). Recommended concentration: 5ug/ml.
• Expiration Date: 12 months from date of receipt.

orb1248327 (0.3 ug/ml) staining of Human Placenta lysate (35 ug protein in RIPA buffer). Detected by chemiluminescence.

orb1248327 (5 ug/ml) staining of paraffin embedded Human Liver. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.

orb1248327 (5 ug/ml) staining of paraffin embedded Human Cortex. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.