ENPP2 / AUTOTAXIN Antibody

• Catalog Number: orb1248327
• Category: Antibodies
• Description: ENPP2 / AUTOTAXIN Antibody
• Target: ENPP2 / AUTOTAXIN
• Clonality: Polyclonal
• Species/Host: Goat
• Conjugation: Unconjugated
• Reactivity: Human
• Form/Appearance: Liquid
• Concentration: 500 ug/mL
• Buffer/Preservatives: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
• Immunogen: The immunogen for this antibody is: PDHLTSCVRPDVRVS
• UniProt ID: Q13822
• MW: Approx 100kDa
• Tested applications: ELISA, IHC, WB
• Application notes: Peptide ELISA: antibody detection limit dilution 1:128000.Western Blot:Approx 100kDa band observed in Human Placenta lysates (calculated MW of 105kDa according to NP_006200.3). Recommended concentration: 0.3-1ug/ml. Primary incubation was 1 hour.Immunohistochemistry:Paraffin embedded Human Liver and Brain (Cortex). Recommended concentration: 5ug/ml.
• Antibody Type: Primary Antibody
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Alternative names: ENPP2, ectonucleotide pyrophosphatase/phosphodiest
• Note: For research use only
• NCBI: NP_001124335.1, NP_001035181.1, NP_006200.3

orb1248327 (0.3 ug/ml) staining of Human Placenta lysate (35 ug protein in RIPA buffer). Detected by chemiluminescence.

orb1248327 (5 ug/ml) staining of paraffin embedded Human Liver. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.

orb1248327 (5 ug/ml) staining of paraffin embedded Human Cortex. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.