ENPP2 Antibody (Center)

• Catalog Number: orb1931393
• Category: Antibodies
• Description: Affinity Purified Rabbit Polyclonal Antibody (Pab)
• Target: This ENPP2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 377-406 amino acids from the Central region of human ENPP2.
• Clonality: Polyclonal
• Species/Host: Rabbit
• Isotype: Rabbit IgG
• Conjugation: Unconjugated
• Reactivity: Human
• Form/Appearance: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
• UniProt ID: Q13822
• MW: 98994 Da
• Tested applications: FC, WB
• Dilution range: WB: 1:1000, WB: 1:1000, WB: 1:2000, WB: 1:2000, FC: 1:10~50
• Clone Number: RB18117
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles
• Alternative names: Ectonucleotide pyrophosphatase/phosphodiesterase f
• Note: For research use only
• NCBI: NP_006200.3, NP_001035181.1, NP_001124335.1

ENPP2 Antibody (Center) flow cytometric analysis of MDA-MB435 cells (right histogram) compared to a negative control cell (left histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

Western blot analysis of (arrow) using rabbit polyclonal ENPP2 Antibody (Center). 293T cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the gene.

Anti-ENPP2 Antibody (Center) at 1:2000 dilution + Human brain lysate. Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size: 99 kDa. Blocking/Dilution buffer: 5% NFDM/TBST.

Anti-ENPP2 Antibody (Center) at 1:2000 dilution + Human brain lysate. Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size: 99 kDa. Blocking/Dilution buffer: 5% NFDM/TBST.

Western blot analysis of lysates from human placenta and plasma tissue lysate (from left to right), using ENPP2 Antibody (Center). Diluted at 1:1000 at each lane. A goat anti-rabbit IgG H&L (HRP) at 1:5000 dilution was used as the secondary antibody. Lysates at 35 ug per lane.