ENPEP Antibody

• Catalog Number: orb1247997
• Category: Antibodies
• Description: ENPEP Antibody
• Species/Host: Goat
• Clonality: Polyclonal
• Tested applications: ELISA, IHC, WB
• Predicted Reactivity: Bovine, Canine, Mouse, Porcine, Rat
• Reactivity: Human
• Immunogen: The immunogen for this antibody is: C-EQYQKTSLAQEKEK
• Concentration: 500 ug/mL
• Dilution range: Peptide ELISA: antibody detection limit dilution 1:64000.Western Blot:Approx 170kDa band observed in Human Brain and Testis lysates (calculated MW of 109kDa according to NP_001968.2). The observed molecular weight corresponds to earlier findings in literature with different antibodies (Ando et al, J Clin Endocrinol Metab. 2002 May;87(5):2303-9; PMID: 11994381). Recommended concentration: 1-3ug/ml.Immunohistochemistry:In paraffin embedded Human Kidney shows staining of basement membrane and the mesangium in the glomerulus. Recommended concentration: 3-5ug/ml.
• Form/Appearance: Liquid
• Conjugation: Unconjugated
• Target: ENPEP
• UniProt ID: Q07075
• NCBI: NP_001968.3
• Storage: Aliquot and store at -20°C. Minimize freezing and thawing.
• Buffer/Preservatives: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
• Alternative names: aminopeptidase A, ENPEP, glutamyl aminopeptidase (
• Note: For research use only
• Application notes: Peptide ELISA: antibody detection limit dilution 1:64000.Western Blot:Approx 170kDa band observed in Human Brain and Testis lysates (calculated MW of 109kDa according to NP_001968.2). The observed molecular weight corresponds to earlier findings in literature with different antibodies (Ando et al, J Clin Endocrinol Metab. 2002 May;87(5):2303-9; PMID: 11994381). Recommended concentration: 1-3ug/ml.Immunohistochemistry:In paraffin embedded Human Kidney shows staining of basement membrane and the mesangium in the glomerulus. Recommended concentration: 3-5ug/ml.
• Expiration Date: 12 months from date of receipt.

orb1247997 staining (1 ug/ml) of Human Testis lysate (RIPA buffer, 30 ug total protein per lane). Primary incubated for 1 hour. Detected by chemiluminescence.

orb1247997 (3.8 ug/ml) staining of paraffin embedded Human Kidney. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.