eNOS (phospho-Ser1177) antibody

• Catalog Number: orb6519
• Category: Antibodies
• Description: eNOS (phospho-Ser1177) antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: FC, WB
• Predicted Reactivity: Bovine, Canine, Guinea pig, Porcine, Rabbit, Rat, Sheep
• Reactivity: Human, Mouse
• Isotype: IgG
• Immunogen: KLH conjugated Synthesised phosphopeptide derived from mouse eNOS around the phosphorylation site of Ser1177 (TQ(p-S)FS)
• Concentration: 1mg/ml
• Dilution range: WB=1:500-2000, Flow-Cyt=2μg /Test, IHC-P=1:100-500, IHC-F=1:100-500, IF=1:100-500, ELISA=1:5000-10000
• Form/Appearance: Liquid
• Conjugation: Unconjugated
• MW: 133 kDa
• Target: Phospho-eNOS (Ser1177)
• UniProt ID: P29474
• Storage: Shipped at 4°C. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.
• Buffer/Preservatives: 0.01M TBS(pH7.4) with 1% rAlbumin, 0.03% Proclin300 and 50% Glycerol.
• Alternative names: eNOS (phospho S1177); eNOS(phospho S1177); p-eNOS
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

References

Lan, Wenya et al. Human urine kininogenase attenuates balloon-induced intimal hyperplasia in rabbit carotid artery through transforming growth factor β1/Smad2/3 signaling pathway J Vasc Surg, 64, 1074-1083 (2016)

PubMed: 26054589

Applications

WB

Reactivity

Rabbit

Hoetzenecker, K. et al. Secretome of apoptotic peripheral blood cells (APOSEC) attenuates microvascular obstruction in a porcine closed chest reperfused acute myocardial infarction model: role of platelet aggregation and vasodilation Basic Res Cardiol, 107, 292 (2012)

PubMed: 22899170

Applications

IHC

Reactivity

Porcine

Western blot analysis of Embryo (Mouse) Lysate using eNOS (phospho-Ser1177) antibody.

Flow cytometric analysis of HepG 2 cells using eNOS (phospho-Ser1177) antibody.