ENO1 Antibody

• Catalog Number: orb1263818
• Category: Antibodies
• Description: ENO1 Antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: FC, IF, IHC-P, WB
• Predicted Reactivity: Bovine, Gallus, Monkey, Rat, Xenopus
• Reactivity: Human, Mouse
• Isotype: Rabbit Ig
• Immunogen: This ENO1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 178-205 amino acids from the Central region of human ENO1.
• Concentration: batch dependent
• Dilution range: For WB starting dilution is: 1:1000For IHC-P starting dilution is: 1:10~50For FACS starting dilution is: 1:10~50For IF starting dilution is: 1:10~50
• Form/Appearance: Liquid
• Conjugation: Unconjugated
• MW: 47 kDa
• Target: ENO1
• UniProt ID: P06733
• NCBI: P06733
• Storage: Store at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
• Buffer/Preservatives: Supplied in PBS with 0.09% (W/V) sodium azide.
• Alternative names: Alpha-enolase, 2-phospho-D-glycerate hydro-lyase,
• Note: For research use only
• Application notes: For WB starting dilution is: 1:1000For IHC-P starting dilution is: 1:10~50For FACS starting dilution is: 1:10~50For IF starting dilution is: 1:10~50
• Expiration Date: 12 months from date of receipt.

Western blot analysis in Jurkat, MCF-7, U251, mouse NIH/3T3 cell line and mouse brain tissue lysates (35 ug/lane).

Formalin-fixed and paraffin-embedded human lymph reacted with ENO1 Antibody, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining.

Flow cytometric analysis of Hela cells (right histogram) compared to a negative control cell (left histogram). FITC-conjugated donkey-anti-rabbit secondary antibodies were used for the analysis.

Fluorescent confocal image of C2C12 cell stained with ENO1 Antibody.C2C12 cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.1%, 10 min), then incubated with ENO1 primary antibody (1:25). For secondary antibody, Alexa Fluor 488 conjugated donkey anti-rabbit antibody (green) was used (1:400).Cytoplasmic actin was counterstained with Alexa Fluor 555 (red) conjugated Phalloidin (7 units/ml). Nuclei were counterstained with DAPI (blue) (10 ug/ml, 10 min). ENO1 immunoreactivity is localized to Cytoplasm significantly.