PDI Antibody

SKU: orb99079

Description

Mouse Anti-Human EndoPDI Monoclonal IgG2a

Images & Validation

• Tested Applications: ELISA, ICC, IF, IHC, IP, WB
• Dilution range: WB (1:200)
• Reactivity: Human
• Application Notes:
  1 µg/ml of SMC-204 was sufficient for detection of EndoPDI in 20 µg of heat shocked HeLa cell lysate by colorimetric immunoblot analysis using Goat anti -mouse IgG:HRP as the secondary antibody.

Key Properties

• Host: Mouse
• Clonality: Monoclonal
• Isotype: IgG2a
• Clone No.: 2E7/7
• Immunogen: Synthesized peptide - 12 amino acids long (ADGEDGQDPHSK) corresponding to residues 52 - 63 of the EndoPDI protein
• Target: PDI
• Molecular Weight: 48kDa
• Purification: Protein G Purified

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: PBS pH 7.4, 50% glycerol, 0.09% sodium azide *Storage buffer changes when conjugated
• Concentration: 1 mg/ml
• Disclaimer: For research use only

Alternative Names

Thioredoxin domain containing 5, ERP46, UNQ364, MGC3178, FLJ21353, FLJ90810, thioredoxin related protein, endothelial protein disulphide isomeras

Immunocytochemistry/Immunofluorescence analysis using Mouse Anti-EndoPDI Monoclonal Antibody, Clone 2E7/7. Tissue: Cervical cancer cell line (HeLa). Species: Human. Fixation: 4% Formaldehyde for 15 min at RT. Primary Antibody: Mouse Anti-EndoPDI Monoclonal Antibody at 1:100 for 60 min at RT. Secondary Antibody: Goat Anti-Mouse ATTO 488 at 1:100 for 60 min at RT. Counterstain: DAPI (blue) nuclear stain at 1:5000 for 5 min RT. Localization: Nucleus, Endoplasmic Reticulum, Endoplasmic Reticulum Lumen. Magnification: 60X.

Quick Database Links

Gene Symbol

PDI

UniProt

Q8NBS9

NCBI Gene ID

81567

RefSeq (Protein):NP_001139021