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Catalog Number | orb148135 |
---|---|
Category | Antibodies |
Description | Mouse monoclonal to EndoPDI (ATTO-488). Endothelial protein disulfide isomerase (EndoPDI) is a thioredoxin member of the protein disulfide isomerase family of chaperones. This enzyme has been localized to the endoplasmic reticulum in primary endothelial cells, but colocalizes with nucleoli in the nuclei of breast, colon, and renal cancer cells. EndoPDI mRNA and protein expression is induced by hypoxia and exhibits a protective effect on endothelial cells during hypoxia.. |
Species/Host | Mouse |
Clonality | Monoclonal |
Clone Number | 2E7/7 |
Tested applications | ELISA, ICC, IHC, IP, WB |
Reactivity | Human |
Isotype | IgG2a |
Immunogen | Synthesized peptide - 12 amino acids long (ADGEDGQDPHSK) corresponding to residues 52 - 63 of the EndoPDI protein |
Concentration | 1 mg/ml |
Dilution range | WB (1:200) |
Conjugation | ATTO-488 |
MW | 48kDa |
Target | PDI |
Entrez | 81567 |
UniProt ID | Q8NBS9 |
NCBI | NP_001139021 |
Storage | Conjugated antibodies should be stored at 4°C |
Buffer/Preservatives | PBS pH 7.4, 50% glycerol, 0.09% sodium azide *Storage buffer may change when conjugated |
Alternative names | Thioredoxin domain containing 5; ERP46; UNQ364; MG Read more... |
Note | For research use only |
Application notes | 1 µg/ml of SMC-204 was sufficient for detection of EndoPDI in 20 µg of heat shocked HeLa cell lysate by colorimetric immunoblot analysis using Goat anti -mouse IgG:HRP as the secondary antibody. |
Expiration Date | 12 months from date of receipt. |
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