ENaC beta antibody

• Catalog Number: orb152739
• Category: Antibodies
• Description: Rabbit polyclonal to ENaC Beta (RPE). The Epithelial Sodium Channel (ENaC) is a membrane ion channel permeable to Na+ ions. It is located in the apical plasma membrane of epithelia in the kidneys, lung, colon, and other tissues where it plays a role in trans epithelial Na+-ion transport. Specifically Na+ transport via ENaC occurs across many epithelial surfaces, and plays a key role in regulating salt and water absorption. ENaCs are composed of three structurally related subunits that form a tetrameric channel, alpha, beta , and gamma. The expression of its alpha and beta subunits is enhanced as keratinocytes differentiate. The beta and gamma-ENaC subunits are essential for edema fluid to exert its maximal effect on net fluid absorption by distal lung epithelia(5). And it has been concluded that the subunits are differentially expressed in the retina of mice with ocular hypertension, therefore the up-regulation of alpha-ENaC proteins could serve as a protection mechanism against elevated intraocular pressure..
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: ICC, IHC, IP, WB
• Reactivity: Frog, Hamster, Human, Mouse, Rat
• Immunogen: Produced against the C-terminal tail (amino acids 617-638) of rat beta ENaC (antibody designation 3755-2)
• Concentration: 1 mg/ml
• Dilution range: WB (1:1000), IHC (1:100)
• Conjugation: RPE
• MW: 87kDa
• Target: ENaC
• Entrez: 24767
• UniProt ID: P37090
• NCBI: NP_036780
• Storage: Conjugated antibodies should be stored at 4°C
• Buffer/Preservatives: PBS, 50% glycerol, 0.09% sodium azide *Storage buffer may change when conjugated
• Alternative names: SCNN1B antibody, Amiloride sensitive sodium channe
• Note: For research use only
• Application notes: 1 µg/ml of SPC-404 was sufficient for detection of beta-ENaC in 20 µg of rat kidney tissue lysate by colorimetric immunoblot analysis using Goat anti-rabbit IgG:HRP as the secondary antibody.
• Expiration Date: 12 months from date of receipt.