Embelin

SKU: orb1300727

Description

Embelin, also known as embelic acid, is a natural compound derived from Ardisia japonica that acts as an inhibitor of the X-linked inhibitor of apoptosis protein (XIAP) with an IC50 of 4.1 µM. This small molecule is utilized in biochemical research to study apoptosis mechanisms and has been investigated in both cellular and animal models for its potential in oncology research.

Research Area

Cell Biology, Immunology & Inflammation, Metabolism Research, Pharmacology & Drug Discovery, Signal Transduction

Key Properties

• CAS Number: 550-24-3
• MW: 294.39
• Purity: 99.60%
• Formula: C₁₇H₂₆O₄
• SMILES: CCCCCCCCCCCC1=C(O)C(=O)C=C(O)C1=O
• Target: Autophagy,Prostaglandin Receptor,Lipoxygenase,IAP,Apoptosis,NF-κB
• Solubility: 10% DMSO+90% Corn Oil:2 mg/mL (6.79 mM);Ethanol:2.9 mg/mL (9.85 mM);DMSO:257.5 mg/mL (874.69 mM)

Bioactivity

• Target IC50:
  X linked:4.1 μM
• In Vitro:
  Embelin from the Japanese Ardisia herb is a small-molecular inhibitor that binds to the XIAP BIR3 domain with which Smac and caspsase-9 bind. Embelin inhibits cell growth of both PC-3 and LNCap cells in a dose-dependent manner, with IC50 values of 3.7 and 5.7 μM. While, the toxicity of Embelin in normal PrEC and in WI-38 cells is much lower with IC50 values of 20.1 μM and 19.3 μM. Treatment of PC-3 cells with 25 and 50 μM of embelin for 48 h, 30% and 75% of cells undergo apoptosis, representing approximately 3-and 9-fold increase as compared to untreated cells. Embelin also potently suppresses the biosynthesis of eicosanoids by selective inhibition of 5-lipoxygenase (5-LO) and Microsomal prostaglandin E2 synthase-1 (mPGES-1) with IC50 = 0.06 and 0.2 mM, respectively.
• Cell Research:
  Cell growth is determined by the MTT-based assay using Cell Proliferation Reagent WST-1 according to the manufacturer's instruction. Cells (5000 cells/well) are grown in medium with 10% FBS, and various concentrations of Embelin are added to the cells. Four to five days later, WST-1 is added to each well and incubated for 1-3 h at 37 °C. Absorbance is measured with a plate reader at 450 nm with correction at 650 nm.(Only for Reference)

Storage & Handling

• Storage: keep away from direct sunlight | Powder: -20°C for 3 years | In solvent: -80°C for 1 year | Shipping with blue ice/Shipping at ambient temperature.
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

cell, carcinoma, cancer, Apoptosis, Autophagy, 5-Lipoxygenase, NSC 91874, Nuclear factor-κB, Nuclear factor-kappaB, NSC91874, NSC-91874, NFkB, NF-kB, NFκB, NF-κB, mPGES-1, inhibit, nonpeptidic, IAP, Inhibitor, growth, Emberine, Embelic acid, Embelin, death, XIAP, squamous, prostate, Prostaglandin Receptor, ProstaglandinReceptor

Compound Identifiers

PubChem CID

3218